Source
Name: European Bioinformatics Institute
pubmed 14681455
psi-mi MI:0469
postalAddress Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SD, United Kingdom
url http://www.ebi.ac.uk

ExperimentList
Experiment #2
Name: mizuno-1999-2
Description: Molecular architecture of the mouse DNA polymerase alpha-primase complex.
pubmed 10523676
Interaction detection method:
Name: anti bait coip :anti bait coimmunoprecipitation
psi-mi MI:0006
pubmed 7708014
Participant identification method:
Name: monoclonal antibody
psi-mi MI:0072
pubmed 14755292
author-list Mizuno T., Yamagishi K., Miyazawa H., Hanaoka F.
contact-email fhanaoka@imcb.osaka-u.ac.jp
figure-legend 3D, 4D, 5C
exp-modification Using an IRES derived from poliovirus, plasmids were designed to coexpress p180 and p68 and also p54 and p46. These are then suitable for the transfection of three or four subunits simultaneously because all of the cells expressing p180 will contain p68 and all of the cells expressing p54 will contain p46 at constant ratios.
Experiment #18
Name: mizuno-1999-3
Description: Molecular architecture of the mouse DNA polymerase alpha-primase complex.
pubmed 10523676
Interaction detection method:
Name: pull down
psi-mi MI:0096
pubmed 14755292
Participant identification method:
Name: western blot (Immuno blot)
psi-mi MI:0113
pubmed 14755292
author-list Mizuno T., Yamagishi K., Miyazawa H., Hanaoka F.
contact-email fhanaoka@imcb.osaka-u.ac.jp
figure-legend 4B, 5B
exp-modification His-tagged proteins pulled down with TALON cobalt-chelating Sepharose resin.
exp-modification Using an IRES derived from poliovirus, plasmids were designed to coexpress p180 and p68 and also p54 and p46. These are then suitable for the transfection of three or four subunits simultaneously because all of the cells expressing p180 will contain p68 and all of the cells expressing p54 will contain p46 at constant ratios.
Experiment #26
Name: mizuno-1999-1
Description: Molecular architecture of the mouse DNA polymerase alpha-primase complex.
pubmed 10523676
Interaction detection method:
Name: fluorescence imaging :fluorescence microscopy
psi-mi MI:0416
pubmed 14755292
Participant identification method:
Name: antibody detection :identification by antibody
psi-mi MI:0421
pubmed 14755292
author-list Mizuno T., Yamagishi K., Miyazawa H., Hanaoka F.
contact-email fhanaoka@imcb.osaka-u.ac.jp
figure-legend 1C, 2B, 2C, 3B
exp-modification Using an IRES derived from poliovirus, plasmids were designed to coexpress p180 and p68 and also p54 and p46. These are then suitable for the transfection of three or four subunits simultaneously because all of the cells expressing p180 will contain p68 and all of the cells expressing p54 will contain p46 at constant ratios.
Experiment #54
Name: mizuno-1999-4
Description: Molecular architecture of the mouse DNA polymerase alpha-primase complex.
pubmed 10523676
Interaction detection method:
Name: cosedimentation
psi-mi MI:0027
pubmed 14755292
Participant identification method:
Name: western blot (Immuno blot)
psi-mi MI:0113
pubmed 14755292
author-list Mizuno T., Yamagishi K., Miyazawa H., Hanaoka F.
contact-email fhanaoka@imcb.osaka-u.ac.jp
figure-legend 7A

InteractorList
Interactor #4
Name: dpola_mouse :DNA polymerase alpha catalytic subunit (gene name: Pola) (gene name synonym: Pola1)
uniprotkb (SP_46) P33609 (dpola_mouse)
interpro IPR006172 (DNA_pol_B)
interpro IPR006133 (DNA_pol_B_exo)
interpro IPR006134 (DNA_pol_B_region)
interpro IPR004578 (Pol2)
interpro IPR012337 (RNaseH_fold)
intact EBI-688051 (dpola_mouse)
Name: protein
psi-mi MI:0326
pubmed 14755292
so SO:0000358
Organism:
Name: mouse :Mus musculus
Sequence: MAPMHEEDCKLEASAVSDSGSFAASRARREKKSKKGRQEA
LERLKKAKAGEKYKYEVEDLTSVYEEVDEEQYSKLVQARQ
DDDWIVDDDGIGYVEDGREIFDDDLEDDALDTCGKGSDGK
AHRKDRKDVKKPSVTKPNNIKAMFIASAGKKTTDKAVDLS
KDDLLGDILQDLNTETAQITPPPVLIPKKKRSTGALLNPF
SVHTPKAIPSGKPASPVLRNEPLLTPIPLKRAELAGELAQ
PECPEDEQELGVMEFEDGDFDESMDTEKVDEKPVTAKTWD
QETEPVERVEHEADPERGTTSYLENFLPDVSCWDIDQDDE
SIPQEVQVDSSNLPLVKGADDEQVFQFYWLDAYEDPYNQP
GVVFLFGKVWIESVKTHVSCCVMVKNIERTLYFLPREMKF
DLNTGKETAIPVTMKDVYEEFDSKISAKYKIMKFKSKIVE
KNYAFEIPDVPEKSEYLEVRYSAEVPQLPQNLKGETFSHV
FGTNTSSLELFLMNRKIKGPCWLEVKNPQLLNQPISWCKF
EVMALKPDLVNVIKDVSPPPLVVMSFSMKTMQNVQNHQHE
IIAMAALVHHSFALDKAPPEPPFQTHFCVVSKPKDCIFPC
DFKEVISKKNMKVEIAATERTLIGFFLAKVHKIDPDILVG
HNICSFELEVLLQRINECKVPYWSKIGRLRRSNMPKLGSR
SGFGERNATCGRMICDVEISAKELIHCKSYHLSELVQQIL
KTERIVIPTENIRNMYSESSYLLYLLEHIWKDARFILQIM
CELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAF
YENNYIVPDKQIFRKPQQKLGDEDEEIDGDTNKYKKGRKK
ATYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICF
TTVQRVTSEVQKATEDEEQEQIPELPDPNLEMGILPREIR
KLVERRKQVKQLMKQQDLNPDLVLQYDIRQKALKLTANSM
YGCLGFSYSRFYAKPLAALVTYKGREILMHTKDMVQKMNL
EVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLYKLL
EIDIDAVFKSLLLLKKKKYAALVVEPTSDGNYITKQELKG
LDIVRRDWCDLAKDTGNFVIGQILSDQSRDTIVENIQKRL
IEIGENVLNGSVPVSQFEINKALTKDPQDYPDRKSLPHVH
VALWINSQGGRKVKAGDTVSYVICQDGSNLTATQRAYAPE
QLQKLDNLAIDTQYYLAQQIHPVVARICEPIDGIDAVLIA
LWLGLDSTQFRVHQYHKDEENDALLGGPAQLTDEEKYKDC
EKFKCLCPSCGTENIYDNVFEGSGLDMEPSLYRCSNVDCK
VSPLTFMVQLSNKLIMDIRRCIKKYYDGWLICEEPTCCSR
LRRLPLHFSRNGPLCPVCMKAVLRPEYSDKSLYTQLCFYR
YIFDADCALEKLTEHEKDKLKKQFFPLRVLQDYRKVKNIA
EQFLSWSGYSEVNLSKLFANYAGKS
Interactor #8
Name: pri1_mouse :DNA primase small subunit (gene name: Prim1)
uniprotkb (SP_44) P20664 (pri1_mouse)
interpro IPR012192 (DNA_prim_euk)
interpro IPR002755 (DNA_primase_S)
intact EBI-848742 (pri1_mouse)
Name: protein
psi-mi MI:0326
pubmed 14755292
so SO:0000358
Organism:
Name: mouse :Mus musculus
Sequence: MEPFDPAELPELLKLYYRRLFPYAQYYRWLNYGGVTKNYF
QHREFSFTLKDDIYIRYQSFNNQSELEKEMQKMNPYKIDI
GAVYSHRPNQHNTVKLGAFQAQEKELVFDIDMTDYDDVRR
CCSSADICSKCWTLMTMAMRIIDRALKEDFGFKHRLWVYS
GRRGVHCWVCDESVRKLSSAVRSGIVEYLSLVKGGQDVKK
KVHLNEKVHPFVRKSINIIKKYFEEYALVGQDILENKENW
DKILALVPETIHDELQRGFQKFHSSPQRWEHLRKVANSSQ
NMKNDKCGPWLEWEVMLQYCFPRLDVNVSKGVNHLLKSPF
SVHPKTGRISVPIDFHKVDQFDPFTVPTISAICRELDMVS
THEKEKEENEADSKHRVRGYKKTSLAPYVKVFEQFLENLD
KSRKGELLKKSDLQKDF
Interactor #11
Name: dpoa2_mouse :DNA polymerase alpha subunit B (gene name: Pola2)
uniprotkb (SP_34) P33611 (dpoa2_mouse)
interpro IPR007200 (DNA_pol_alpha_B)
intact EBI-848759 (dpoa2_mouse)
Name: protein
psi-mi MI:0326
pubmed 14755292
so SO:0000358
Organism:
Name: mouse :Mus musculus
Sequence: MAVSTQQLAEELQIFGLDYEDSLLEKLAELCVLYRQTEDG
MVSELIAFCTSAGKTCLTVDILNSFEYEVLNKKLSKAWHS
ASKDSGHAGTRDIVSIQELIEAEEEEETLLSSYTTPSKGP
LKRVSSTPETPLTKRSVAARSPRQLLSPSSFSPSATPSQK
YTSRTNRGEVVTTFGSAQGLSWSGRGGSGSVSLKVVGDPE
PLTGSYKAMFQQLMGVREVLTSKIEELGSELKEHHKIEAF
TPLLVPAQEPVILLGQIGCDSNGKLNSKSVILEGDQEHSY
GAQIPVDLSELKEYSLFPGQVVIMEGFNTTGRRLTATKLY
EGVPLPFYQPTEEEGASEQTMVVVARGPYTTSDSITYDPL
LDLIAIINRDQPDVCILFGPFLDAKHEQVENCKLTSPFED
VFKQCLRTVIEGTRSSGSHLVFVPSLRDVHHEPVYPQPPF
TFSELSREDKKRVQFVSEPCSLSINGVMFGLTSTDLLFHI
GAEEIFSSSGTSDRFSRVLKHILTQRSYYPLYPPHEDMAI
DYENFYTYAQLPVTPDVFIVPSELRYFVKDIFGCVCVNPG
RLTKGQVGGTFGRLYLRRQPKAMDGGGRQGLSVAAQVVRI
Interactor #28
Name: pri2_mouse :DNA primase large subunit (gene name: Prim2)
uniprotkb (SP_35) P33610 (pri2_mouse)
interpro IPR007238 (DNA_primase_lrg)
intact EBI-848748 (pri2_mouse)
Name: protein
psi-mi MI:0326
pubmed 14755292
so SO:0000358
Organism:
Name: mouse :Mus musculus
Sequence: MQFSGRIRKKLRLAGDQRNASYPHSLQFYLQPPTENISLT
EFENLAFDRVKLLKAIENLGVSYVKGTEQYQSKLEAEIRK
LKFSYRENLEDEYEPRRRDHISHFILRLAYCQSEDLRRWF
IQQEMDLLRFRFSILPKDKVQSFLKDSHLHFEAISDEEKT
LREQDIMASSPSLSGIKLESESVYKVPFADALDLFRGRKV
YLEDGFAYVPLKDIVAIILNEFRATLSKALALTARSLPAV
QSDERLQPLLNHLSHSYTGQDYSTQKNTGKISLDQIDSLS
TKSFPPCMRQLHKALRENHHLRHGGRMQYGLFLKGIGLTL
EQALQFWKQEFIRGKMDPDKFDKGYSYNIRHSFGKEGKRT
DYTPFSCMKIILTNPPGQGDYHGCPFRHSDAELLKQKMQS
YKIPASGISQILDLVKGNHYQVACQKYFEMTHNVDDCGFS
LNHPNQFFFESQRILTGGKDIKKEISQPETPQHKPSTQKT
RDAASALASLDSSLEMDLEGLEEYF

InteractionList
Interaction #1
Name: pola-prim1-6 :Interaction between murine Prim1 and Pola detected by coimmunoprecipitation
intact EBI-848860 (pola-prim1-6)
Experiments: 2
Participant #3
Interactor #4
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: prey
psi-mi MI:0498
pubmed 14755292
Participant #7
Interactor #8
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: bait
psi-mi MI:0496
pubmed 14755292
Interaction Type:
Name: physical interaction (aggregation)
psi-mi MI:0218
pubmed 14755292
kd 1.0
Interaction #9
Name: prim1-pola2-1 :Interaction between murine Prim1 and Pola2 detected by coimmunoprecipitation
intact EBI-848931 (prim1-pola2-1)
Experiments: 2
Participant #10
Interactor #11
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: prey
psi-mi MI:0498
pubmed 14755292
Participant #12
Interactor #8
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: bait
psi-mi MI:0496
pubmed 14755292
Participant #14
Interactor #8
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: prey
psi-mi MI:0498
pubmed 14755292
Interaction Type:
Name: physical interaction (aggregation)
psi-mi MI:0218
pubmed 14755292
comment P33610 co-expressed in this experiment
kd 1.0
Interaction #17
Name: pola-pola2-4 :Interaction between murine Pola and Pola2 detected by pulldown
intact EBI-848912 (pola-pola2-4)
Experiments: 18
Participant #19
Interactor #11
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: prey
psi-mi MI:0498
pubmed 14755292
Participant #20
Interactor #4
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: bait
psi-mi MI:0496
pubmed 14755292
Interaction Type:
Name: physical interaction (aggregation)
psi-mi MI:0218
pubmed 14755292
kd 1.0
Interaction #25
Name: prim2-prim1-1 :Interaction between murine Prim1 and Prim2 detected by colocalisation
intact EBI-848753 (prim2-prim1-1)
go GO:0000060 (protein import into nucleus, t)
Experiments: 26
Participant #27
Interactor #28
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: neutral component
psi-mi MI:0497
pubmed 14755292
Participant #29
Interactor #8
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: neutral component
psi-mi MI:0497
pubmed 14755292
Interaction Type:
Name: colocalization
psi-mi MI:0403
pubmed 14755292
kd 1.0
Interaction #31
Name: pola-pola2-1 :Interaction between murine Pola and Pola1 detected by colocalisation
intact EBI-848764 (pola-pola2-1)
go GO:0000060 (protein import into nucleus, t)
Experiments: 26
Participant #32
Interactor #4
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: neutral component
psi-mi MI:0497
pubmed 14755292
Participant #33
Interactor #11
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: neutral component
psi-mi MI:0497
pubmed 14755292
Interaction Type:
Name: colocalization
psi-mi MI:0403
pubmed 14755292
kd 1.0
Interaction #34
Name: pola-pola2-2 :Interaction between murine Prim2, Pola and Pola1 detected by colocalisation
intact EBI-848769 (pola-pola2-2)
go GO:0000060 (protein import into nucleus, t)
Experiments: 26
Participant #35
Interactor #4
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: neutral component
psi-mi MI:0497
pubmed 14755292
Participant #36
Interactor #11
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: neutral component
psi-mi MI:0497
pubmed 14755292
Participant #37
Interactor #28
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: neutral component
psi-mi MI:0497
pubmed 14755292
Interaction Type:
Name: colocalization
psi-mi MI:0403
pubmed 14755292
kd 1.0
Interaction #38
Name: pola-pola2-3 :Interaction between murine Prim1, Prim2, Pola and Pola2 detected by colocalisation
intact EBI-848775 (pola-pola2-3)
go GO:0000060 (protein import into nucleus, t)
go GO:0005658 (alpha DNA polymerase:primase c)
Experiments: 26
Participant #39
Interactor #4
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: neutral component
psi-mi MI:0497
pubmed 14755292
Participant #40
Interactor #11
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: neutral component
psi-mi MI:0497
pubmed 14755292
Participant #41
Interactor #8
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: neutral component
psi-mi MI:0497
pubmed 14755292
Participant #42
Interactor #28
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: neutral component
psi-mi MI:0497
pubmed 14755292
Interaction Type:
Name: colocalization
psi-mi MI:0403
pubmed 14755292
kd 1.0
Interaction #43
Name: pola-prim1-4 :Interaction between murine Prim1, Prim2, and Pola detected by colocalisation
intact EBI-848828 (pola-prim1-4)
go GO:0005737 (cytoplasm)
Experiments: 26
Participant #44
Interactor #4
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: neutral component
psi-mi MI:0497
pubmed 14755292
Participant #45
Interactor #8
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: neutral component
psi-mi MI:0497
pubmed 14755292
Participant #46
Interactor #28
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: neutral component
psi-mi MI:0497
pubmed 14755292
Interaction Type:
Name: colocalization
psi-mi MI:0403
pubmed 14755292
kd 1.0
Interaction #47
Name: pola-prim1-5 :Interaction between murine Prim1, Prim2, and Pola detected by colocalisation
intact EBI-848834 (pola-prim1-5)
go GO:0005737 (cytoplasm)
Experiments: 26
Participant #48
Interactor #4
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: neutral component
psi-mi MI:0497
pubmed 14755292
Participant #51
Interactor #28
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: neutral component
psi-mi MI:0497
pubmed 14755292
Participant #52
Interactor #8
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: neutral component
psi-mi MI:0497
pubmed 14755292
Interaction Type:
Name: colocalization
psi-mi MI:0403
pubmed 14755292
kd 1.0
Interaction #53
Name: pola-pola2-5 :Interaction between murine Prim1, Prim2, Pola and Pola2 detected by colocalisation
intact EBI-848981 (pola-pola2-5)
go GO:0000060 (protein import into nucleus, t)
go GO:0005658 (alpha DNA polymerase:primase c)
Experiments: 54
Participant #55
Interactor #4
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: neutral component
psi-mi MI:0497
pubmed 14755292
Participant #56
Interactor #8
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: neutral component
psi-mi MI:0497
pubmed 14755292
Participant #57
Interactor #28
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: neutral component
psi-mi MI:0497
pubmed 14755292
Participant #58
Interactor #11
Biological Role:
Name: unspecified
psi-mi MI:0499
pubmed 14755292
Experimental Role:
Name: neutral component
psi-mi MI:0497
pubmed 14755292
Interaction Type:
Name: physical interaction (aggregation)
psi-mi MI:0218
pubmed 14755292
kd 1.0